Journal: International Journal of Immunopathology and Pharmacology

Article Title: MiR-1298 affects cell proliferation and apoptosis in C6 cells by targeting SET domain containing 7

doi: 10.1177/0394632017720546

Figure Lengend Snippet: Rno-miR-1298 was down-regulated in C6 cells. The relative expression of rno-miR-1298 was examined in C6 cells by qRT-PCR. The Ct value of rno-miR-1298 expression exceeds 35, indicating the abundance of rno-miR-1298 expression was low in C6 cells.

Article Snippet: Reverse transcription was carried out using M-MLV reverse transcriptase (Promega, Madison, USA). rno-miR-1298 primer was purchased from RiboBio (Guangzhou, China).

Techniques: Expressing, Quantitative RT-PCR

Journal: International Journal of Immunopathology and Pharmacology

Article Title: MiR-1298 affects cell proliferation and apoptosis in C6 cells by targeting SET domain containing 7

doi: 10.1177/0394632017720546

Figure Lengend Snippet: The lentiviral vector of rno-miR-1298 was successfully constructed and expressed: (a) the lentiviral vector of rno-miR-1298 was expressed in cell lines C6 and (b) the expression of rno-miR-1298 was evaluated by qRT-PCR after the lentiviral vector of rno-miR-1298 infected C6 cells. The expression of rno-miR-1298 was obviously increased in C6 cells. *** P < 0.001.

Article Snippet: Reverse transcription was carried out using M-MLV reverse transcriptase (Promega, Madison, USA). rno-miR-1298 primer was purchased from RiboBio (Guangzhou, China).

Techniques: Plasmid Preparation, Construct, Expressing, Quantitative RT-PCR, Infection

Journal: International Journal of Immunopathology and Pharmacology

Article Title: MiR-1298 affects cell proliferation and apoptosis in C6 cells by targeting SET domain containing 7

doi: 10.1177/0394632017720546

Figure Lengend Snippet: rno-miR-1298 suppressed cell proliferation. The effect of rno-miR-1298 on proliferation of C6 cells was detected by MTT. The results indicated that overexpression of rno-miR-1298 significantly inhibited proliferation of C6 cells and the inhibited ratio was significant at fourth and fifth days. * P < 0.05, ** P < 0.01.

Article Snippet: Reverse transcription was carried out using M-MLV reverse transcriptase (Promega, Madison, USA). rno-miR-1298 primer was purchased from RiboBio (Guangzhou, China).

Techniques: Over Expression

Journal: International Journal of Immunopathology and Pharmacology

Article Title: MiR-1298 affects cell proliferation and apoptosis in C6 cells by targeting SET domain containing 7

doi: 10.1177/0394632017720546

Figure Lengend Snippet: rno-miR-1298 induced cell apoptosis: (a, b) flow cytometry assays the effect of (b) rno-miR-1298 and (a) negative control on apoptosis of C6 cells; (c) statistical analysis of apoptosis indicated that rno-miR-1298 could obviously induced apoptosis of C6 cells. * P < 0.05.

Article Snippet: Reverse transcription was carried out using M-MLV reverse transcriptase (Promega, Madison, USA). rno-miR-1298 primer was purchased from RiboBio (Guangzhou, China).

Techniques: Flow Cytometry, Negative Control

Journal: International Journal of Immunopathology and Pharmacology

Article Title: MiR-1298 affects cell proliferation and apoptosis in C6 cells by targeting SET domain containing 7

doi: 10.1177/0394632017720546

Figure Lengend Snippet: SETD7 was a direct target of rno-miR-1298: (a) sequence alignment of wild-type (WT) and mutated (MT) putative rno-miR-1298 binding sites in the 3′-UTR of SETD7, (b) the expression of SETD7 was examined after infection of rno-miR-1298 in C6 cells by western blot, and (c) relative luciferase activities of plasmids were detected in cells expressed WT or MT of SETD7 3′-UTR. rno-miR-1298 significantly inhibited the luciferase activity of the WT compared with NC. ** P < 0.01.

Article Snippet: Reverse transcription was carried out using M-MLV reverse transcriptase (Promega, Madison, USA). rno-miR-1298 primer was purchased from RiboBio (Guangzhou, China).

Techniques: Sequencing, Binding Assay, Expressing, Infection, Western Blot, Luciferase, Activity Assay

Journal: International Journal of Immunopathology and Pharmacology

Article Title: MiR-1298 affects cell proliferation and apoptosis in C6 cells by targeting SET domain containing 7

doi: 10.1177/0394632017720546

Figure Lengend Snippet: rno-miR-1298 targeted SETD7 to affect cellular proliferation and apoptosis: (a) knockdown of SETD7 had obviously suppressive effects on cell proliferation from the fourth day in C6 cells, (b, c) flow cytometry showed that knockdown of SETD7 significantly induced cell apoptosis of C6 cells (b) compared to negative control (c), and (d) statistical analysis of apoptosis in C6 cells. ** P < 0.01.

Article Snippet: Reverse transcription was carried out using M-MLV reverse transcriptase (Promega, Madison, USA). rno-miR-1298 primer was purchased from RiboBio (Guangzhou, China).

Techniques: Flow Cytometry, Negative Control